The chip giant's market capitalization reaches $3.335 trillion as share prices continue their stellar rise.
Nvidia, the startup at the center of the artificial intelligence boom, has become the most valuable company in the world, knocking Microsoft out of the top spot.
Nvidia's market capitalization reached $3.335 trillion on Tuesday, as the chipmaker's shares rose 3.5 percent to $135.58.
The feat comes just days after the Santa Clara, California-based company eclipsed Apple to become the second most valuable company in the world.
Shares of Microsoft and Apple, which occupy the No. 2 and No. 3 spots, fell 0.45 percent and 1.1 percent, respectively.
Nvidia's rally, which has lifted the S&P 500 and Nasdaq indexes to record levels, continues a speculator winning streak for the company, whose graphics processing units (GPUs) are integral to AI development.
Driven by voracious demand for its chips from tech giants like Microsoft, Meta and Google, the company's share price is up nearly 182 percent this year alone, after tripling in 2023.
Nvidia controls about 80 percent of the market for AI chips used in data centers needed to run AI models like OpenAI's ChatGPT.
Since its stock market debut in 1999, Nvidia shares have soared 591,078 percent.
An investor who invested $10,000 in the company in 1999 would have $59,107,800 in shares today, according to the capital markets newsletter Kobeissi Letter.
Nvidia spent its first few decades focusing primarily on producing computer gaming chips.
But during the 2000s, CEO Jensen Huang directed the company to invest heavily in developing GPUs for use in applications beyond gaming, preparing it to capitalize on the rise of AI.
The company's astonishing rise has made Huang one of the richest men in the world, with an estimated net worth of more than $117 billion, according to Forbes.
